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c flag pcdna3  (Addgene inc)


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    Structured Review

    Addgene inc c flag pcdna3
    C Flag Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+flag+pcdna3/c-Flag+pcDNA3+(Plasmid+%2320011)/pmc12990383-95-0-3
    Average 95 stars, based on 78 article reviews
    c flag pcdna3 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver.
    Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011, deposited by Dr. Smale)25 and pN3-3×Flag-Control to construct Flag-Nwd1-N and 3 × Flag-Nwd1-C (hereafter called Flag-Nwd1-C). .. To construct Halo-Nwd1, full-length Nwd1 cDNA was subcloned into the pFN21A-HaloTag-CMV Flexi-vector (Promega, Madison, WI, USA).

    Article Title: Selective disruption of Traf1/cIAP2 interaction attenuates inflammatory responses and rheumatoid arthritis.
    Article Snippet: The human TRAF1 ORF was amplified from RAJI cells cDNA and cloned into pENTR-D-TOPO vector (Invitrogen) following manufacturer ‘s instructions then used as the template plasmid to generate all the different mutations. .. The TRAF1 mutated plasmids were sub-cloned into c-Flag pcDNA3. c-Flag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20011) [37]; Flag-cIAP2/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 27973) [38]; pEBB HA cIAP1 was a gift from Colin Duckett (Addgene plasmid # 38232) [39]. ..

    Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver
    Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011, deposited by Dr. Smale) and pN3-3×Flag-Control to construct Flag-Nwd1-N and 3 × Flag-Nwd1-C (hereafter called Flag-Nwd1-C). .. To construct Halo-Nwd1, full-length Nwd1 cDNA was subcloned into the pFN21A-HaloTag-CMV Flexi-vector (Promega, Madison, WI, USA).

    Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways.
    Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, c-Flag pcDNA3 (Addgene plasmid # 20011, kindly provided by Stephen Smale). ..

    Construct:

    Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver.
    Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011, deposited by Dr. Smale)25 and pN3-3×Flag-Control to construct Flag-Nwd1-N and 3 × Flag-Nwd1-C (hereafter called Flag-Nwd1-C). .. To construct Halo-Nwd1, full-length Nwd1 cDNA was subcloned into the pFN21A-HaloTag-CMV Flexi-vector (Promega, Madison, WI, USA).

    Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver
    Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into C-Flag-pcDNA3 (Addgene, plasmid # 20011, deposited by Dr. Smale) and pN3-3×Flag-Control to construct Flag-Nwd1-N and 3 × Flag-Nwd1-C (hereafter called Flag-Nwd1-C). .. To construct Halo-Nwd1, full-length Nwd1 cDNA was subcloned into the pFN21A-HaloTag-CMV Flexi-vector (Promega, Madison, WI, USA).

    Amplification:

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.
    Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCT TATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCA TCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (Addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder HiFi DNA assembly Master mix (New England Biolabs). .. Site-directed mutagenesis for amino acid substitution (cysteine to alanine at position 11, C11A) was performed using the Q5 Site-directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instruction.

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis
    Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCTTATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCATCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (Addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder HiFi DNA assembly Master mix (New England Biolabs). .. Site-directed mutagenesis for amino acid substitution (cysteine to alanine at position 11, C11A) was performed using the Q5 Site-directed mutagenesis kit (New England Biolabs) according to the manufacturer's instruction.

    Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways.
    Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, c-Flag pcDNA3 (Addgene plasmid # 20011, kindly provided by Stephen Smale). ..

    Polymerase Chain Reaction:

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis.
    Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCT TATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCA TCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (Addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder HiFi DNA assembly Master mix (New England Biolabs). .. Site-directed mutagenesis for amino acid substitution (cysteine to alanine at position 11, C11A) was performed using the Q5 Site-directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instruction.

    Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis
    Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCTTATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCATCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (Addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder HiFi DNA assembly Master mix (New England Biolabs). .. Site-directed mutagenesis for amino acid substitution (cysteine to alanine at position 11, C11A) was performed using the Q5 Site-directed mutagenesis kit (New England Biolabs) according to the manufacturer's instruction.

    Expressing:

    Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways.
    Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, c-Flag pcDNA3 (Addgene plasmid # 20011, kindly provided by Stephen Smale). ..



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