c flag pcdna3 (Addgene inc)
95
Structured Review
Addgene inc
c flag pcdna3
C Flag Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+flag+pcdna3/c-Flag+pcDNA3+(Plasmid+%2320011)/pmc12990383-95-0-3
Average 95 stars, based on 78 article reviews
C Flag Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+flag+pcdna3/c-Flag+pcDNA3+(Plasmid+%2320011)/pmc12990383-95-0-3
Average 95 stars, based on 78 article reviews
c flag pcdna3 - by Bioz Stars,
2026-09
95/100 stars
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Plasmid Preparation:Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver. Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into Article Title: Selective disruption of Traf1/cIAP2 interaction attenuates inflammatory responses and rheumatoid arthritis. Article Snippet: The human TRAF1 ORF was amplified from RAJI cells cDNA and cloned into pENTR-D-TOPO vector (Invitrogen) following manufacturer ‘s instructions then used as the template plasmid to generate all the different mutations. .. The TRAF1 mutated plasmids were sub-cloned into Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways. Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, Construct:Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver. Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver Article Snippet: .. Nwd1 cDNA corresponding to the N-terminal or C-terminal region of the protein (accession number BC082552; 4–1026 bp for the N-terminus and 2578–4563 bp for the C-terminus) was subcloned into Amplification:Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis. Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCT TATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCA TCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCTTATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCATCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways. Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, Polymerase Chain Reaction:Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis. Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCT TATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCA TCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in Article Title: Peroxisomal import stress activates integrated stress response and inhibits ribosome biogenesis Article Snippet: Human PEX5 cDNA was purchased from Dharmacon Mammalian Gene Collection. .. The hPEX5 was amplified by PCR using the forward and reverse primers (5′-CACTATAGGGAGACCCAAGCTTATCTAGACATGGCAATGCGGGAGCT-3′ and 5′-TCTTACTTGTCATCGTCGTCCTTGTAGTCGCCCTGGGGCAGGCC-3′) and introduced between XhoI and BamHI sites in Expressing:Article Title: E53, E96, D162, E247 and D322 in Ca 2+ -binding domains of annexin A2 are essential for regulating intracellular [Ca 2+ ] and crystal adhesion to renal cells via ERK1/2 and JNK signaling pathways. Article Snippet: The full-length ANXA2 WT was amplified from cDNA isolated from MDCK cells using the PCR method with Q5 High-Fidelity DNA Polymerase (New England Biolabs) and a specific primer pair. .. The primers used in full-length amplification were designed by incorporating cutting sites for HindIII and BamHI in forward and reverse primers, respectively (Supplementary Table S1) for ligating into a mammalian expression vector, |